Troubleshooting and practical help for chromatographers

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CHROMacademy Newsletter
23 August 2016

Biopharmaceuticals Quiz

Test your knowledge on biopharmaceuticals with our "just for fun" quiz.

How to Choose a Stationary Phase for HILIC

HILIC requires a hydrophilic stationary phase to adsorb a water layer in order to allow the analyte partitioning process to take place.  The hydrophilicity of the column packing material will influence the thickness to the water layer which will directly impact analyte retention...

Technical Tip - Useful free tools for HPLC method development

Tony Taylor brings you a round up of some really useful HPLC method development resources from around the internet.

HILIC, IEX, and SEC for the Analysis of Biomolecules

As well as the amino acid composition it is important to characterize the glycosylation of proteins; including the sequence, composition, branching, linkage etc.

5 ways to improve your Split / Splitless Injection technique

1. Understand the process...
Split/splitless injectors typically vaporize a sample dissolved in a suitable organic solvent under increased temperature.  The sample vapors are entrained into the carrier gas flow inside a ‘liner’ or ‘sleeve’ within the inlet...