Troubleshooting and practical help for chromatographers
No Images? Click here
Test your knowledge on biopharmaceuticals with our "just for fun" quiz.
HILIC requires a hydrophilic stationary phase to adsorb a water layer in order to allow the analyte partitioning process to take place. The hydrophilicity of the column packing material will influence the thickness to the water layer which will directly impact analyte retention...
Tony Taylor brings you a round up of some really useful HPLC method development resources from around the internet.
As well as the amino acid composition it is important to characterize the glycosylation of proteins; including the sequence, composition, branching, linkage etc.
1. Understand the process...
Split/splitless injectors typically vaporize a sample dissolved in a suitable organic solvent under increased temperature. The sample vapors are entrained into the carrier gas flow inside a ‘liner’ or ‘sleeve’ within the inlet...