The CHROMacademy Essential Guide to...

Choosing the Correct Column and Mobile Phase for LC‑MS Characterization of Biologics

On-Demand Webcast

View now at http://www.Chromacademy.com/LC-MS-method-development-for-biologics.html

KEY LEARNING OBJECTIVES

Given the heterogeneity and complexity of biologics, including monoclonal antibodies, advance analytical techniques are now essential for characterization, identification, and quantitation. Reversed phase liquid chromatography with mass spectrometry (RP‑MS) is the primary tool for confirmation of intact mass and characterization of post translational modifications (PTMs), such as glycosylation, oxidation, and deamidation. The use of MS detection adds another dimension to method development, as not only is it essential that the method has the capability of resolving the critical pairs, but it must also be accomplished using mobile phases and columns that are compatible with the detector. We will provide you with practical hints and tips to help select the optimum reversed phase column and choose the mobile phase which will achieve the desired analytical goal. This webcast will serve as your reference guide for RP‑MS method development.

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