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LC�MS-MS Method for the Rapid Analysis of Five Artificial Sweeteners Using a Core Enhanced Technology Column Eilidh MacRitchie and Kim Phipps, Thermo Fisher Scientific Some sweeteners do not have a UV chromophore, therefore, detection at the required limits using HPLC-UV can be a challenge. This application note demonstrates an alternative approach using LC�MS-MS with a Thermo Scientific� Accucore� RP-MS HPLC column for rapid analysis of five artificial sweetners. Comparative Analysis of Cooking Oils Using a Solid Core HPLC Column Mark Tracy and Derek Hillbeck, Thermo Fisher Scientific The use of Thermo Scientific� Accucore� C30 columns for the characterization of different cooking oils is demonstrated. The C30 phase offers greater selectivity and resolution of minor components when compared to the C18 phase. Determination of Catechins and Phenolic Acids in Red Wine by Solid Phase Extraction and HPLC Monica Dolci, Thermo Fisher Scientific This application note demonstrates a simple and rapid HPLC method for the analysis of nine catechins and phenolic acids in red wine. Extraction of these polar analytes was achieved on Thermo Scientific� HyperSep� Retain PEP material. The polyphenols in the extracts were quantified by a matrix-standard calibration, with extracts from a wine sample spiked with increasing amounts of analytes. Determination of the polyphenols was performed by HPLC, using a Thermo Scientific� Accucore� PFP HPLC column under gradient mobile phase conditions.

Analysis of Cephalosporins on a Polar Endcapped C18 3 �m Particle Column Valeria Barattini, Thermo Fisher Scientific The analysis of species containing polar groups using reversed phase HPLC can be challenging. The polar groups present on the analytes can result in secondary interactions with silanols on the silica surface. Furthermore, the overall polarity of the molecule sometimes calls for the use of mobile phases with very high aqueous content to ensure the analyte solubility, retention, and elution, at the expense of stationary phase stability. In this application note we demonstrate the analysis of a mix of four analytes from the cephalosporin antibiotics family on a Thermo Scientific� Syncronis� aQ 3 �m particle column. The analysis yields excellent peak shape and resolution in short run times, as well as outstanding reproducibility. An Improved HPLC Method for the Separation of Norethindrone and Mestranol with Progesterone as Internal Standard Derek Hillbeck, Thermo Fisher Scientific This work demonstrates the use of a Thermo Scientific� Syncronis� 3 �m C8 column to produce effective separation of norethindrone, progesterone, and mestranol giving equivalent results to those required from the USP method but in less time. Analysis of Non-Steroidal Anti-Inflammatory Drugs Using a Highly Pure, High Surface Area C18 HPLC Column Jamil Ali, Thermo Fisher Scientific This application note demonstrates the use of the Thermo Scientific� Syncronis� C18 HPLC column for the analysis of non-steroidal anti-inflammatory drugs. Syncronis C18 columns provide a fast simple method with good hydrophobic retention, excellent peak shape, and high resolution.